Assembly of a G-Quadruplex Repair Complex by the FANCJ DNA Helicase and the REV1 Polymerase
نویسندگان
چکیده
منابع مشابه
Human Rev1 polymerase disrupts G-quadruplex DNA
The Y-family DNA polymerase Rev1 is required for successful replication of G-quadruplex DNA (G4 DNA) in higher eukaryotes. Here we show that human Rev1 (hRev1) disrupts G4 DNA structures and prevents refolding in vitro. Nucleotidyl transfer by hRev1 is not necessary for mechanical unfolding to occur. hRev1 binds G4 DNA substrates with Kd,DNA values that are 4-15-fold lower than those of non-G4 ...
متن کاملG-quadruplex recognition and remodeling by the FANCJ helicase
Guanine rich nucleic acid sequences can form G-quadruplex (G4) structures that interfere with DNA replication, repair and RNA transcription. The human FANCJ helicase contributes to maintaining genomic integrity by promoting DNA replication through G4-forming DNA regions. Here, we combined single-molecule and ensemble biochemical analysis to show that FANCJ possesses a G4-specific recognition si...
متن کاملFANCJ promotes replication of G-quadruplex DNA structures
Thank you again for submitting your manuscript EMBOJ-2014-88663, "FANCJ promotes replication of G-quadruplex DNA structures." We have now received all the reports from three experts, which I am enclosing copied below. As you will see, the reviewers express some interest in the basic finding of your study, yet they also raise a number of substantive concerns, and issues with the potential to con...
متن کاملHuman Pif1 helicase is a G-quadruplex DNA-binding protein with G-quadruplex DNA-unwinding activity.
Pif1 proteins are helicases that in yeast are implicated in the maintenance of genome stability. One activity of Saccharomyces cerevisiae Pif1 is to stabilize DNA sequences that could otherwise form deleterious G4 (G-quadruplex) structures by acting as a G4 resolvase. The present study shows that human Pif1 (hPif1, nuclear form) is a G4 DNA-binding and resolvase protein and that these activitie...
متن کاملFANCJ promotes DNA synthesis through G-quadruplex structures
Our genome contains many G-rich sequences, which have the propensity to fold into stable secondary DNA structures called G4 or G-quadruplex structures. These structures have been implicated in cellular processes such as gene regulation and telomere maintenance. However, G4 sequences are prone to mutations particularly upon replication stress or in the absence of specific helicases. To investiga...
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ژورنال
عنوان ژورنال: Genes
سال: 2019
ISSN: 2073-4425
DOI: 10.3390/genes11010005